Tests Performed

Test Code

Samples Needed

Aerobic Bacterial Culture

Rhodococcus equi included               

AER

- A swab inoculated with sample and placed in aerobic transport media (Amies without charcoal).

- Transtracheal wash (TTW) (preferred).

Anaerobic Bacterial Culture              

ANAER

- A swab inoculated with tracheal wash fluid and placed in anaerobic transport media.

- Fresh lung (>2-3 cm in diameter, minimum size).

- Swab of lung or piece of lung tissue aseptically collected and immediately placed in anaerobic transport media.

Equine Respiratory PCR                   

ERPNL

- Nasal or pharyngeal swabs in viral transport media or a red top tube with 0.5ml sterile saline

-  Transtracheal wash (TTW) (preferred)

Fecal Floatation         

FLOAT

-  Fresh feces in leak-proof container.

Fibrinogen (heat-precipitation)

FIB-SCH

-  EDTA whole blood

Fungal Culture                       

FUNGCM

-  Swab of transtracheal wash (TTW) (preferred sample) or swab of bronchoalveolar lavage (BAL) fluid in anaerobic transport media or Amies transport media.

-  Swab of lung or piece of lung tissue in anaerobic transport media or Amies transport media

Hemogram, Equine                

LA CBC

- (2) air dried blood smears.

-  EDTA whole blood (lavender top tube).

Immunoglobulin G, Equine - TIA

EQUINE IGG

-  Serum (separated).

Rhodococcus equi virulence PCR           

REQUPCR     

-  Transtracheal wash (TTW) (preferred).

Tracheal Wash Cytology           

TWE  

-  Two air dried slides of fluid.

-  Transtracheal wash (TTW) (preferred).