This guideline primarily focuses on the recommendations for evaluating the efficiency and safety of Assisted Oocyte Activation (AOA) in Intracytoplasmic Sperm Injection (ICSI) to attain the most favourable reproductive and offspring health results. Additionally, it intends to analyse various evidence-based protocols and techniques that can be employed during AOA in ICSI. Additionally, it scrutinises numerous procedures that ought to be abandoned due to the lack of evidence substantiating their efficacy.